Apple's i Tunes media player tracks how many times a particular audio file has been played.
This is displayed in a column labeled "Play Count" in a user's i Tunes library.
Apple developed the play count feature of i Tunes to help with their "Smart Playlists" feature.
Smart Playlists allows users to control the way the "Shuffle" feature in i Tunes chooses music.
Right-click on the "Play Count" column of the song in your i Tunes library and select "Reset Play Count." You can also reset the play count of your entire i Tunes library by selecting all of the files instead of just one.